Zhang Lei*, Yang Feng, Zhu Junzhe
Background: Luteinizing hormone (LH) plays a vital role in induced ovulation and controlled ovarian hyperstimulation in human assisted reproduction. This evaluation has a brief review of the roles of LH in supporting follicular development and function of LH Ovulation Rapid Test Cassette in preparing pregnancy.
A rapid increase in LH plasma, called an LH surge, induces ovulation within 2-3 days. The surge can be detected by the observation of LH in serum or urine with ovulation predictor kits (OPKs). If a woman knows the duration of her menstrual cycle, she can plan the timing of testing. The average length of the menstrual cycle is 28 days, but a regular cycle lasting anywhere between 24 and 38 days is considered normal. The cycle length is determined by follicular growth and by the lifespan of the corpus luteum [1]. Many women experience varying cycle lengths with back and forth shifts in the day of ovulation, which may pose a problem especially for infertility patients. To detect ovulation, patients are required to determine LH levels in urine with an OPK daily until getting a positive result, which causes undue stress in addition to the financial burden.
To optimize the probability of conception in a menstrual cycle, the appropriate timing of intercourse is of utmost importance. Randomized controlled trials show evidence that ovulation predictor kits (OPKs) may increase pregnancy rates. In ovulatory cycles, ovulation usually occurs about 14 days before the onset of the next period. The length of the normal ovulatory cycle may vary considerably (26-35 days, mean 28 days), and extensive variations both in follicular (10-23 days) and luteal phases (7-19 days) [2]. Thus, making the prediction of ovulation and appropriate timing for intercourse or natural cycle intrauterine insemination is rather challenging.
Objective: The main purpose of this evaluation report was to explore the reliability and performance of the Citest LH Ovulation Rapid Test Cassette for the qualitative detection of of luteinizing hormone (LH) in urine to aid in the detection of ovulation.
Method: Run a rapid chromatographic immunoassay for the qualitative detection of LH in urine to aid in the detection of ovulation, containing the anti-LH particles and anti-LH antibody coated on the membrane.
Result: The results show that the overall relative sensitivity for the detection of LH Ovulation Rapid Test Cassette (Urine) is 40mIU/ml, and the accuracy is 99.0%.
Conclusion: Citest LH ovulation Rapid Test Cassette (Urine) is a rapid test that utilizes a rapid chromatographic immunoassay for the qualitative detection of luteinizing hormone (LH) in urine to aid in the detection of ovulation.
The product is simple to operate, gives results within 5 minutes of the sample being tested and has high accuracy. Women can use this test kit to obtain accurate results and to determine the ideal time to get pregnant.
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